Применение клатратобразующего газа для разработки способа сберегающей криоконсервации стволовых клеток

Abstract

A new cryopreservation method using xenon, a clathrate-forming gas, under normal pressure was investigated. The objective of the study was to determine whether this cryostasis protocol could protect stem cells at cryogenic temperatures and whether it has advantages over the standard protocol using DMSO. The results provided the first evidence that a cryostasis protocol using xenon as a clathrate-forming gas under normal pressure may have protective effects on stem cells. It was shown that xenon, in contrast to DMSO, does not cause unwanted differentiation of stem cells, and, in addition, allows for greater preservation of stem cells during cryopreservation. This cryostasis technology may find applications in developing new approaches for long-term cryopreservation protocols.